Detection and quantification of pathogenic microorganisms in patients with chronic periodontitis before and after treatment with chlorhexidine digluconate

The aim of this study was to evaluate the efficiency chlorhexidine digluconate solution (CHX) as an adjunct nonsurgical therapy (NPT) patients with chronic periodontitis (CP), by analyzing presence and quantity periopathogenic microorganisms in subgingival biofilm. DNA extracted from biofilm obtained 40 CP divided into two groups (NPT+CHX NPT alone control) at baseline 2 months after therapy. selected periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, E. corrodens, T. denticola, forsythia) determined polymerase chain reaction (PCR), while total bacterial load assessed quantitative PCR. incidence decreased following treatment, both NPT+CHX alone, but without reaching statistically significant difference group. In group, a reduction prevalence species: denticola (P = 0.008) forsythia 0.016), well microorganism count 0.002) observed treatment. conclusion, present findings support use CHX adjunctive CP.